OglyZOR® Smart Enzymes

OglyZOR endoglycosidase (O-Glycosidase) specifically hydrolyzes O-glycans of core 1 and to some extent core 3 disaccharides on native glycoproteins. The novel OglyZOR enzyme technology is highly efficient and easy to use compared to other similar enzymes on the market.

Product Description

OglyZOR is an endoglycosidase (endo-α-N-acetylgalactosaminidase) that catalyzes the hydrolysis of core 1 and to some extent core 3 O-glycans (disaccharides) from native glycoproteins. The sialic acids of the glycans should be removed for OglyZOR enzyme activity. The SialEXO, composed of a mix of sialidases for high efficiency in hydrolyzing α2-3, α2-6 or α2-8 bonds, is used together with OglyZOR for efficient removal of the O-linked disaccharides. Optimal activity is obtained at pH 6.5 to 7.5 and at 37 °C.

 

OglyZOR is derived from Streptococcus oralis and expressed in Ecoli. The enzyme contains a His-tag and the molecular weight is 227 kDa. SialEXO is derived from Akkermansia muciniphila and expressed in Ecoli. The SialEXO-enzymes contain His-tags and the molecular weights of the components are 42 kDa and 66 kDa, respectively.

 

Unit Definition

One unit digests ≥ 90% of O-glycans of 1 µg glycoprotein (TNFαR) when incubated together with one unit of SialEXO in 20 mM Tris pH 6.8 at 37 °C for 2 h.

 

Content and Storage

The box includes:

1 vial of OglyZOR (G1-OG1-020) supplied lyophilized in TBS pH 7.6, with no preservatives added.

1 vial of SialEXO (G1-SM1-020) supplied lyophilized in TBS pH 7.6, with no preservatives added.

 

The box is shipped cold and should be stored at –20 °C upon arrival.
After reconstitution, the enzymes are stable for 1 month at +4-8 °C.

 

The enzyme is supplied as a lyophilized powder and is available in 2000 unit vials for deglycosylation of 2 mg glycoprotein. It requires removal of sialic aids for activity, and is therefore provided with 2000 units SialEXO® (sialidase).

 OglyZOR Product box

 

Key Characteristics:

Hydrolyzes O-glycans on glycoproteins

2-4h reaction

Requires removal of sialic acids using SialEXO® (included)

Core 1 type O-glycan disaccharides

 

 

For more information about OglyZOR® Smart Enzymes