OglyZOR is an endoglycosidase (endo-α-N-acetylgalactosaminidase) that catalyzes the hydrolysis of core 1 and to some extent core 3 O-glycans (disaccharides) from native glycoproteins. The sialic acids of the glycans should be removed for OglyZOR enzyme activity. The SialEXO, composed of a mix of sialidases for high efficiency in hydrolyzing α2-3, α2-6 or α2-8 bonds, is used together with OglyZOR for efficient removal of the O-linked disaccharides. Optimal activity is obtained at pH 6.5 to 7.5 and at 37 °C.
OglyZOR is derived from Streptococcus oralis and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 227 kDa. SialEXO is derived from Akkermansia muciniphila and expressed in E. coli. The SialEXO-enzymes contain His-tags and the molecular weights of the components are 42 kDa and 66 kDa, respectively.
Unit Definition
One unit digests ≥ 90% of O-glycans of 1 µg glycoprotein (TNFαR) when incubated together with one unit of SialEXO in 20 mM Tris pH 6.8 at 37 °C for 2 h.
Content and Storage
The box includes:
1 vial of OglyZOR (G1-OG1-020) supplied lyophilized in TBS pH 7.6, with no preservatives added.
1 vial of SialEXO (G1-SM1-020) supplied lyophilized in TBS pH 7.6, with no preservatives added.
The box is shipped cold and should be stored at –20 °C upon arrival.
After reconstitution, the enzymes are stable for 1 month at +4-8 °C.
