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IgGZERO (EndoS) is an IgG-specific endoglycosidase acting on complex type N-glycans at the Fc-glycosylation site of IgG. The enzyme acts on the chitobiose core and leaves the core GlcNAc intact.
IgGZERO (EndoS) is an endoglycosidase from Streptococcus pyogenes that specifically hydrolyzes N-linked glycans in the Fc-region of native IgG. It can be used to rapidly deglycosylate antibodies to reduce sample complexity, or to inactivate Fc-mediated effector functions.
The Enzyme deglycosylates all human IgG subclasses, and IgGs from many different species, including mouse, rat, monkey, sheep, goat, cow, and horse. The enzyme only hydrolyzes glycans from the Fc-domain of IgG, even when other glycosylated proteins are present in the solution. The enzyme is expressed in E. coli, contains a His-tag, and the molecular weight is 110 kDa.
The IgGZERO enzyme has limited activity on high-mannose and hybrid-type glycans (Sjögren et al., 2015). If you are interested in endoglycosidases that hydrolyze all glycoforms on IgG, we recommend the GlycINATOR product family.
Available in three different product formats:
IgGZERO Enzyme – lyophilized enzyme
IgGZERO LE – lyophilized enzyme with low levels of endotoxin
deGlycIT – immobilized on agarose beads
Human IgG1-4, Fc-fusion proteins, IgG from mouse, rat, monkey, sheep, goat, cow and horse
30 min reaction
Requires native IgG fold
Complex Fc glycoforms, limited activity on high-mannose and hybrid-type glycans
For more information about IgGZERO® (EndoS) Smart Enzymes