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GingisREX (Rgp) is an arginine-specific protease that digests proteins C-terminally of arginine residues.
GingisREX is a cysteine protease that specifically digests peptide bonds C-terminally to arginine residues, including arginine linked to proline. Cysteine is required for enzymatic activity.
The enzyme is purified from Porphyromonas gingivalis.
Stability and Storage
The enzyme is provided as a lyophilized powder. After reconstitution by addition of water, GingisREX is stable for at least 1 month at 4-8°C. The product is shipped cold and should be stored at -20°C upon arrival.
Buffers and Reducing Agent
To maintain the enzymatically active cysteine in a reduced form, TCEP is added to preserve the reducing environment (for details please consult the user instructions). GingisREX is active in a broad pH range, 5.0-9.0, with optimal activity between pH 6.5-8.0. Digestion can be performed in the presence of chaotropic agents or detergents such as urea, SDS, tween and SDC. Buffers tested and compatible with GingisREX activity are Tris, Bis-Tris and ammonium bicarbonate.
The GingisREX enzyme is supplied as a lyophilized powder with no preservatives added. It is available in vials of 5 μg enzyme.
Digests any peptide or protein containing arginine. Speci c for Arg-X motifs
60 min reaction
Active in 6 M urea and 0.1% SDS
C-terminally of arginine residues
For more information about GingisREX® Smart Enzymes