FabRICATOR® Z (IdeZ) Smart Enzymes

FabRICATOR Z (IdeZ) is a cysteine protease that digests mouse IgG2a and IgG3 at a specific site below the hinge, generating a homogenous pool of F(ab’)2 and Fc fragments after 2 hours incubation.

Product Description

FabRICATOR Z is an enzyme for preparation of F(ab’)2 from mouse subclasses IgG2a and IgG3. FabRICATOR Z digests mouse IgG2a and IgG3 at a specific site below the hinge region, generating intact F(ab’)2 and Fc fragments. Most mouse IgG2a and IgG3 are digested by FabRICATOR Z within 2 hours, although the enzyme:antibody ratio and/or digestion time may need to be optimized for certain antibodies. For digestion of human IgGs, FabRICATOR® is recommended.

 

FabRICATOR Z digests IgG at a specific site and there is no risk of overdigestion if the incubation time is prolonged. FabRICATOR Z digests IgG at mild conditions, thus preserving the immunoreactivity. FabRICATOR Z digests IgG in sodium phosphate buffer with pH ranging from 6.0-8.0, but is most active at pH 6.5 and at low NaCl concentrations.

 

FabRICATOR Z is derived from S. equi ssp. zooepidemicus and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 36 442 Da.

 

FabRICATOR Z is available in three different product formats:

 

FabRICATOR Z Enzyme – lyophilized enzyme

 

FragIT Z – immobilized on agarose beads

 

FragIT Z kit – immobilized enzyme and affinity purification resin for preparation of F(ab’)2 fragments

product-box-fabricator-z IdeZ

 

Key Characteristics

Mouse IgG2a and IgG3, IgG from monkey, rabbit and sheep

2 h reaction

No need for reducing agents or co-factors

CPAPNLLG / GPSVF (below the hing

 

 

For more information about FabRICATOR® Z (IdeZ) Smart Enzymes