FabRICATOR (IdeS) is a unique enzyme that digests IgG at a specific site below the hinge region, generating a homogenous pool of F(ab’)2 and Fc/2 fragments. No other substrate besides IgG is known. The IgG is digested within 30 minutes, and there is no risk of overdigestion if the incubation time is prolonged. Since FabRICATOR digests IgG under physiological reaction conditions, the immunoreactivity is preserved. The best activity is obtained at 37°C, but digestion can also be performed at room temperature using a prolonged reaction time.
FabRICATOR digests all subclasses of human, monkey, rabbit and sheep IgG. It has limited activity on mouse IgG2a and IgG3 – for digestion of these antibody species, we recommend using FabRICATOR Z.
FabRICATOR is cloned from Streptococcus pyogenes and expressed in E. coli. The enzyme contains a His-tag and has a molecular weight of 37 725 Da. FabRICATOR is a recombinantly modified form of IdeS that has been developed by Genovis AB for biotech applications requiring the highest standards and consistency.
FabRICATOR is available in five different product formats:
FabRICATOR Enzyme – lyophilized enzyme
FragIT – immobilized on agarose beads
FragIT kit – immobilized enzyme and affinity purification resin for generation and purification of F(ab’)2 and Fc/2 fragments from IgG
FabRICATOR LE – lyophilized enzyme with low levels of endotoxins
FabRICATOR Validation kit – consists of three batches of the enzyme to simplify method validation
FabRICATOR-HPLC – immobilized enzyme in an HPLC column for fast online digestion of monoclonal antibodies
Also, a method to detect the FabRICATOR Enzyme is available:
Anti-FabRICATOR – a goat polyclonal antibody directed towards the FabRICATOR enzyme
